Microbial Aerosol Research Laboratory
LLC
10975 Doll Road
Monmouth, OR 97361-9542
Phone/Fax: 503-838-2264
Purpose Personnel
Current Research
Publications Links
Microbial Aerosol Research
Laboratory LLC (MARL) mission statement- MARL’s
mission is to provide consultation and conduct research concerned with
out-of-doors atmospheric microbial (primarily bacteria) populations, all
aspects of their dynamics and interactions with other biota. At present
(i.e., 2001) MARL’s activities include analyses
of atmospheric microbial dynamics in several parts of the world using state-of-the-art
statistical analysis, and the interaction of honey
bees with atmospheric bacterial spores.
E-mail: lighthab@open.org
Personnel:
CEO: Bruce Lighthart, PhD, retired as
a senior microbial ecologist from the EPA after 27 years. He founded
MARL
in 1998.
Senior Research Associate: Kevin R. Steffy
Prier is a research biologist with graduate training in insect neurophysiology.
He has worked in several laboratories in Oregon, France, and Switzerland.
He has been working for MARL since it was
founded in 1998.
Current
Research:
Atmospheric microbial dynamics: A large data set with measurements
of total particle counts, meteorological and air pollution independent
parameters, and dependent biological particle counts measured over 1.5
years at several stations are being analyzed with state-of-the-art statistical
methods to determine a set of “rules” to predict the dependent parameters.
Honey bees as detectors of environmental bacteria:
We are currently studying the theoretical and practical aspects of using
honey bees (Apis mellifera) to collect airborne bacterial spores.
Honey bees acquire an electrostatic charge during flight. This charge
is thought to be involved in pollen collection, as the charge on the bee
attracts particles of opposite charge. It has been our hypothesis
that a flying bee will tend to collect any oppositely charged particulate
material that they fly through, including aerosolized bacterial spores.
We have shown in the laboratory that tethered bees flying in a wind tunnel
adsorb aerosolized bacterial spores and that the quantity of spores adsorbed
is dependent on spore exposure and the electrostatic charge on the bee.
We have further developed a mathematical model that can be used to predict
the quantity of spores a free-flying bee will adsorb when flying through
a known concentration of aerosolized bacterial spores. This work
is supported by a grant from DARPA
through the University of Montana.
Recent publications
from MARL:
-
Lighthart, B., K.R. Prier, G.M. Loper, and J.J. Bromenshenk (2000).
Bees scavenge airborne bacteria. Microb. Ecol. 39:314-321.
PDF,
180Kb
-
Lighthart, B., K.R. Prier, G.M. Loper, and J.J. Bromenshenk (2000). Honey
bees scavenge airborne bacteria from the atmosphere. Proceedings,
14th Conference on Biometeorology & Aerobiology, pp. 233-234.
PDF,
20Kb
-
Prier, K.R., B. Lighthart, G.M. Loper, and J.J. Bromenshenk (2000). Honey
bees scavenge airborne bacteria from the atmosphere. Proceedings,
1st Joint Conference on Point Detection for Chemical and Biological Defense,
pp. 380-389.
PDF,
620Kb
-
Prier, K.R.S., B. Lighthart, and J.J. Bromenshenk (2001). An adsorption
model of aerosolized bacterial spores (Bacillus subtilis variety
niger)
onto free-flying honey bees (Hymenoptera: Apidae) and its validation. Environ.
Entomol. 30:1188-1194.
PDF,
258Kb
-
Lighthart, B., K.R.S. Prier, and J.J. Bromenshenk (2002). Detection
of aerosolized bacterial spores (Bacillus subtilis var. niger)
using free-flying honey bees (Hymenoptera: Apidae) as collectors.
Appl. & Environ. Microbiol. In Review.
Links to
related web sites:
-
Bee-Alert! -
Environmental Hazard Detection and Warning program at the University of
Montana.
-
BeeSource.com - Online sourcebook
for beekeeping.
-
Bee Culture -
"The magazine of American beekeeping."
-
Environmental Entomology -
A journal of the Entomological Society of America.
-
Bioback - Ambient Biobackground
Characterization (requires user registration).
-
DARPA -
Defense Advanced Research Projects Administration, Controlled Biological
Systems division.
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This web site is maintained by Kevin
Prier, MARL. Created 3 May, 2001. Last updated Friday,
8 February, 2002.